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The cell number, defined by count of DAPI stained nuclei on membranes, as well as the measured nuclei area, were not influenced by the application of DON from apical side at any concentration or time in comparison to the control (DON0).
Signal quantification was calculated for each nucleus by determining the nuclei area and quantization of the signal in that area.
Gating for single cells of flat morphology was done based on nuclei area, roundness and intensity of Hoechst in the nucleus and in the cytoplasm.
After cells were treated with ox-LDL, fluorescence staining for NF- κB increased in nuclei area, indicating translocation of NF- κB from cytoplasm into nucleus.
Ten features were calculated from the morphology of the nuclei (area, perimeter, eccentricity, length major axis, length of minor axis, convex area, solidity, equivalent diameter, rectangularity, and compactness).
(C ) The two orange and two blue arrows show the phenotypes (nuclei area and cell number) of the two dsRNAs designed for sti and Cdc23.
Similar(43)
Briefly, DAPI-stained nuclei and GFP-transfected cells were identified using Otsu's method, nuclei areas were subtracted from the identified cells to give a cytoplasmic GFP intensity and this was used to give a nuclear/cytoplasmic intensity ratio for each image.
We developed the ImmunoRatio image analysis software, which segments the DAB- and hematoxylin-stained nuclei areas from a microscope image, calculates the labeling index (percent of DAB-stained area out of the total nuclear area), and generates a pseudo-colored result image matching the segmentation.
Finally, a decrease in number of cell nuclei per area (nuclei density) with a concomitant increase in connective tissue was observed by examination of the H&E stained sections in tumors from mice treated with Irinophore C™.
We also computed the proportion of nuclei (within area groups ) with negative JSDiff of each of the training set cases.
Both the density of nuclei and area of the section were quantified using digital image analysis (ImageJ; U.S. National Institutes of Health; http://rsbweb.nih.gov/ij/).
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