(b) hNSCs fluorescently labelled using Alexa Fluor® 488 Phalloidin (green) to detect actin and Hoechst dye (blue) to visualize nuclei after 7 DIV on Control TCPS, Control Glass H NDs and O NDs, with and without PL + LN coating.
It is remarkable that in all acytokinetic embryos, daughter nuclei after mitosis are arranged in spatially normal positions, and maternal vasa mRNAs accumulate in the prospective planes of the first and second cell cleavages in the total absence of cytokinesis.
The density of nuclei after the CVD process was insufficient for the formation of diamond film in the case of no Co removal by acid, when a scratching pretreatment was adopted.
Experiments performed with a polarized light microscope revealed an increase in the number of activated nuclei after shear treatment, and alignment of these nuclei along flow lines after long shearing times.
v Four nuclei after a meiotic-like division.
The histological sections also showed that Bcl-2 accumulated in the nuclei after radiation exposure (Figure 4).
The red fluorescence was mainly observed in cytoplasm with a small portion in the nuclei after 4 h (Figure 9A).
More than 60% of internalized [125I]SGMIB-MNT radioactivity accumulated in the cell nuclei after a 1-h incubation.
We performed a similar analysis on the EM images of isolated Hela nuclei after high pressure freezing and freeze substitution.
Hoechst 33342 staining indicated apoptotic cells to have shrunken, condensed and fragmented nuclei after exposure of BTB extract for 48 h [Fig. 2c (ii, iii, v, vi)].
The imaging and ET data collection of ultrathin-sectioned nuclei after high-pressure freezing, freeze substitution, and fixation were also done in the FEI Tecnai Spirit.
