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Interestingly, a significant amplification of cellular and nuclear strains was observed in the direction perpendicular to the cell axis.
Assays were run in separate blocks for the inbred versus the outbred nuclear strains.
Separate models were run for the experiments involving the outbred versus the inbred nuclear strains.
In addition to being run separately from one another, the assays involving the outbred versus the inbred nuclear strains were each split into two blocks.
In contrast, offspring produced from crosses involving the isogenic nuclear strains resulted in completely standardized nuclear genotypes, such that all sons from a given cross shared a near identical genotype (Table 1).
The BRO haplotype, which harbours the candidate TFT mutation, consistently exhibited lower noncompetitive fertility relative to controls when expressed alongside haploid copies of nuclear genotypes sourced from the three outbred nuclear strains (F2,8.90 = 17.1, P < 0.001).
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The relationship between micrometer strain and cell and nuclear strain are reported in Figure S1.
Note that the slope of the cell strain curve is about double that for nuclear strain, suggesting that there is incomplete force transmission between the basal parts of the cell and the nucleus and/or the nucleus is stiffer than the rest of the cell.
After blocking, cells were washed thrice with PBS and incubated for 45 min at RT with monoclonal antiperoxiredoxin antibody generated in mouse at 1 : 250 dilution, washed with PBS, and incubated for 30 min with anti-mouse secondary antibody tagged with Alexa-448 in 1 : 500 dilution along with nuclear strain Hoechst 33342 (2.5 μg/mL).
In the mito-nuclear introgression strains, a nuclear background effect on mitochondrial respiratory capacity was observed.
Mito-nuclear introgression strains with Aut nuclear backgrounds displayed stronger flight ability than those with an OreR nuclear background (Fig. 2A).
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