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Nuclear signal enumeration was only performed on cells with clearly defined nuclear borders and clearly visible signals.
A potential downside of the nuclear signal is that it will have decayed away within 100,000 years – a blink of the eye in geological time.
P65 translocation to the nucleus is represented as an increase in nuclear signal in HeLa cells.
PiCdc14 shows a clear nuclear signal, and a weaker punctate cytoplasmic signal.
In HEK293T cells endogenous Gadd45a showed only a weak, homogeneous nuclear signal.
Altogether we conclude that NLS1 constitute a functional, necessary and sufficient nuclear signal.
This is evident by the fact that control animals showed a robust cytoplasmic Arc signal with minimal nuclear signal.
A bright red intracytoplasmic and nuclear signal was observed by means of the avidin-biotin-peroxidase complex method.
By means of the avidin-biotin-peroxidase complex method, a bright red intracytoplasmatic and nuclear signal was observed.
We found that depletion of BRCA1 in both HMEC and MCF7 does not appreciably modify XIST nuclear signal morphology.
For quantification of results, blinded images were analyzed for poly(A) nuclear signal using the ImageJ Cell Counter plugin.
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