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The nuclear section of the survey group has also finished its work and left Iraq.
"Sorry to bombard on this point," he wrote to Scarlett, "but I do worry that the nuclear section will become the main focus and as currently drafted is not in great shape".
This approach prevented oversampling of cells and minimized measurement error due to insufficient pixel number in a nuclear section.
One sample had a 2+ IHC score and 5 copies of the HER2-neu gene per nuclear section; two of the cases with a 3+ IHC score had > 15 copies of the HER2-neu gene per nuclear section (Table 4 and 5).
However, the RR activity of cancer cell nuclear section could not be detected in a cell fractionation study, which indicated that the biological role of RRM2 nuclear localization is largely unknown.
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Altogether these observations show that individual nucleosomes and even connecting DNA can be resolved in the tomograms of stained nuclear sections and that individual, highly extended, chromatin fibers can be depicted in these EC domains.
The real nuclear envelope profile (blue) and PICs (green) positions are thus assessed as illustrated in Fig. 5; panel A illustrates a single x-y section showing three distinct intranuclear PICs while panel B reports adjacent vertical nuclear sections derived from the same dataset.
The total area of the square is 50.625 μm in two fields per section, for a total of 20 fields per block and this permits to obtain the number of nuclear sections of the area (Nav).
DOI: http://dx.doi.org/10.7554/eLife.07759.012 To confirm this loss of proximity between LAP2α and the telomeres in HGPS cells, we quantified the surface area of telomeres co-localizing with LAP2α for each telomere in normal or HGPS nuclear sections.
We then measured the distribution profile of LAP2α along a ∼400 nm axis from the center of each telomere in 3D-SIM nuclear sections (544 nm thick), at 45° angle intervals.
Subsequent investigations determined that the accident began at about 4 00 a.m. with a failure in the non-nuclear section of the plant, which necessitated the opening of a relief valve.
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