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The amino acid sequences from Arabidopsis and Oryza for all five nuclear members of the clpP gene family were aligned using ClustalW version 1.83 [32], with default settings, together with chloroplast clpP1 amino acid sequences.
The final step(s) involved the evolution of the cytosolic and nuclear members upon the specialization of the eukaryotic cell (nucleolus etc).
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The first group is composed of families where at least one parent has migrated, while the second group is limited to families in which no nuclear member has migrated.
The GRs are encoded by a family of genes known as nuclear member subfamily 3 (NR3C) genes.
Interestingly, a second nuclear member was present in the Pezizomycotina fungi that showed orthology to the human parvulin Par14 (Group A).
After siRNA was treated for 48 h, 4% paraformaldehyde was used to fix cells on the slides and 0.1% Triton X-100 was used to break the nuclear member.
Iran's president, Muhammad Khatami, has promised to give the IAEA more information, earlier, about future civilian nuclear plans as other non-nuclear members of the Nuclear Non-Proliferation Treaty already do.
Tomasz Szatkowski said Poland's Defence Ministry was discussing whether to ask for access to Nato's "nuclear-sharing" program, an initiative that allows non-nuclear members of the bloc to borrow nuclear weapons from the United States.
Suicides were significantly more likely to occur in nuclear family members, who tended to be younger (16.4%), than in extended family members, who tended to be older (2.2%), P<0.001.
Backlogs for family-based visas separate even nuclear family members for years.
Its two nuclear power members, Britain and France, have big interests in preserving that status.
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