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The set-up has been designed to couple AGATA with a magnetic spectrometer, charged-particle and neutron detectors, scintillators for the detection of high-energy γ rays and other devices such as a plunger to measure nuclear lifetimes.
Notably, however, Nlrp6 and Mlxipl had substantially longer nuclear lifetimes of 1.98 ± 0.96 hr for Nlrp6 and 0.75 ± 0.37 hr for Mlxipl.
Most export rates were higher than the cytoplasmic degradation rates, and they conformed to previous estimates of nuclear lifetimes of a few minutes.
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Nuclear localization of mRNA does not necessarily imply increased nuclear lifetime, namely, low export rate of mRNA.
It is shown that the secondary-γ emitted from the counterpart nucleus contributes only a small portion to the modification of the nuclear lifetime.
The longer nuclear lifetime indicates that protection from surrounding water molecules is more effective when 1 is bound to DNA.
A nuclear lifetime of 185±12 ns (position a, blue region) is again consistent with a DNA bound complex, as observed in live cells and fixed metaphase spreads.
In formaldehyde-fixed cells under these conditions, 2 displays similar properties to 1 in both cell lines, with nuclear lifetime of 190±10 ns, and a shorter cytoplasmic lifetime of 150±10 ns (Supporting Information, Figure S5 and S6).
So far, the longest reported nuclear singlet lifetimes are 26 minutes[ 3] for the N pair in N2O and 16 minutes for the C pair in some acetylene derivatives[ 10] (both measured in low magnetic field).
We developed a single-molecule in situ technique to quantify nuclear mRNA lifetimes and found that the transcripts of these genes can spend hours in the nucleus before being exported to the cytoplasm.
Currently, nuclear spin order lifetimes exceeding one hour have only been achieved in the gas phase.[ 1] It is much more difficult to achieve long-term storage of nuclear spin order in solution.
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