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These nuclear layers are interspersed by the outer and inner plexiform layers (OPL/IPL) containing the neural connections.
Its retina (Fig. 2c) has a rudimentary appearance, comprising only two main nuclear layers containing the photoreceptors and the output neurons (retinal ganglion cells), respectively.
Sections were counterstained with DAPI to reveal the nuclear layers.
DAPI (Sigma) was also added to locate the nuclear layers of the retina.
The thickness of the nuclear layers were normalized to total retinal thickness at each eccentricity, for both control and UF008/SAP injected eyes.
The vertebrate retina derives from common, multipotent progenitor cells that give rise to six classes of neurons and one type of glial cell organized into three nuclear layers.
The thicknesses of the outer and inner nuclear layers were not diminished compared to controls, suggesting the absence of non-specific cell death.
The cellular organization of Sez-6 null retina and the thickness of the nuclear layers were not apparently different from those of wild-type retina (Fig. 4A & B).
TUNEL staining revealed an increase in the number of apoptotic cells in the ganglion cells layer, the inner, and outer nuclear layers of the retina (Figure 8A).
Analysis of thickness and number of cells in retinal nuclear layers was performed at the inner border of peripheral 1/3 retina by using a microscope (Leica DM RBE, Bensheim, Germany) equipped with an analysis program (Leica IM50, Herrbrugg, Switzerland).
TUNEL assays (Figure 5) indicated that KA promotes apoptotic death of cells in the ganglion cell layer at 24 h, and in both inner nuclear layer and outer nuclear layers at subsequent time periods.
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