Sentence examples for nuclear fractionation from inspiring English sources

Nuclear fractionation was performed and the amount of total β-catenin in the cytoplasmic and nuclear fractions assayed (Fig. 2B).

In an initial pilot experiment, HeLa cells transiently transfected with a β-globin minigene construct βTERM (that has an HIV-LTR promoter) and a plasmid encoding the viral transactivator Tat (pTat), were subjected to UV crosslinking followed by nuclear fractionation into chromatin (Ch) and nucleoplasm (N) fractions, as described previously (West et al., 2008).

Finally, nuclear fractionation studies show cosedimentation of GRWD1 with preribosomal complexes, as well as the WD-repeat-containing protein Bop1, which has previously been implicated in ribosome biogenesis.

Separation of cytosolic and nuclear fractionation was carried as previously described [45].

Nuclear fractionation was carried out according to the manufacturer's protocol (Pierece, IL).

Nuclear fractionation studies were conducted as previously described [28] with slight modifications.

For nuclear fractionations, cells (2 × 10) were fractionated by Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime) according to the manufacturer's instructions.

The nuclear fractionations were done on non-treated cells and cells subjected to 45 °C for 1 h.

To detect potential 2SC targets at low abundance, we performed mitochondrial and nuclear fractionations of Fh1KO MEFs.

More specifically, hyperphosphorylated Gro/TLEs are preferentially recovered in chromatin-enriched nuclear fractions after subcellular fractionation experiments, whereas underphosphorylated forms are found mostly in postnuclear supernatant fractions [27], [31], [38].

In order to obtain pure cytoplasmic or nuclear fraction, Subcellular Protein Fractionation Kit (Thermo Scientific, Waltham, MA, USA) was employed, according to manufacturer's instructions, with 2 × 10 cells per experimental group.