The interaction of telomeres with nuclear domains or compartments greatly differs not only between organisms but also between cells within the same organism.
The mammalian cell nucleus provides a landscape where genes are regulated through their organization and association with freely diffusing proteins and nuclear domains.
From smallest to largest scales: DNA sequence, epigenetic modifications, cis-regulatory elements, DNA looping, TADs (topologically associated domains), and nuclear domains, together control the genome in a coordinated and dynamic manner.
Furthermore, we provide evidence that this region not only mediates the interaction of hr with components of the nuclear architecture, but also specifies the sub-nuclear location of the hr protein to nuclear domains containing deacetylase activity.
DNA damage sensing proteins have been shown to localize to the sites of DNA double strand breaks (DSB) within seconds to minutes following ionizing radiation (IR) exposure, resulting in the formation of microscopically visible nuclear domains referred to as radiation-induced foci (RIF).
Here, we report uptake of silica (SiO2) nanoparticles to the cell nucleus where they induce aberrant clusters of topoisomerase I (topo I) in the nucleoplasm that additionally contain signature proteins of nuclear domains, and protein aggregation such as ubiquitin, proteasomes, cellular glutamine repeat (polyQ) proteins, and huntingtin.
PML is the principle structural constituent of PML nuclear domains, stress responsive polymeric structures that are disrupted by protein aggregates [19], [20].
Similarly, in the present study, HP1α staining was concentrated in several large nuclear domains in MCF7 cells (Fig. 1b, e, h, k&n).
One property of pathogenic ataxin-1 protein is to sequester and disrupt specific nucleoproteins such as promyelocytic leukaemia nuclear domains (PML-NDs) [18], [20], [22].
At first sight, rod photoreceptor nuclei show two distinct nuclear domains that reflect the early structural definition of EC and HC based on regions differentially stained with basophilic dyes [29].
The domains we describe in these young fibers differ from the nuclear domains described in fully differentiated myofibers in that they are not associated with a single nucleus but are, in fact, bounded by nuclei.
