To examine nuclear diameter, cells were stained with Hoechst 33342 dye.
After 1 h of STS (1 μM) incubation, the mean neuritic length and nuclear diameter were both decreased (P<0.01 versus control) (Figures 1a and b).
Kadota, K. et al. Comprehensive pathological analyses in lung squamous cell carcinoma: single cell invasion, nuclear diameter, and tumor budding are independent prognostic factors for worse outcomes.
Six non-overlapped neurites/nuclei were randomly selected from each micrograph, the average neuritic length and nuclear diameter (μm) were calculated from 60 neurites and 60 nuclei, respectively.
The neuritic length and nuclear diameter were measured by ImageJ 1.47e software (Wayne Rasband, National Institutes of Health, Bethesda, MD, USA).
Determinations of nuclear diameter by phase-contrast microscopy, of height by electron microscopy, and of pore frequency by freeze-etching were done on cells during the exponential growth phase (48 h after plating).
Reflectance spectroscopy can probe changes in epithelial nuclei that are important in pre-cancer detection, such as mean nuclear diameter, nuclear size distribution and nuclear refractive index.
Although the nuclear diameter and eccentricity are not observed to change, the light scattering signal reveals a change in the organization of subcellular structures that we interpret using fractal dimension (FD).
Analysis of depth-resolved nuclear morphology data revealed a/LCI was able to detect a significant increase in the nuclear diameter at the depth bin containing the basal layer of the epithelium for dysplastic versus non-dysplastic and HSIL versus LSIL/Benign biopsy sites (both p < 0.001).
In treated group, vitellogenic female liver accompanied with the accumulation of lipid droplets and showed enlarged hepatocytes, increased nuclear diameter and large vacuoles in the cytoplasm (Fig. 1d), whereas the liver of control females had large amount of hepatocyte nuclear polarization, vacuolization in cytoplasm and isolated necrosis (Fig. 1c).
Using Leica LCS software, maximum nuclear diameter was defined as the longest line that crossed the nucleus.
