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In order to make a first investigation of the nuclear damages that could contribute to the induction of autophagic programmed cell death in senescent NHEKs, we performed a Hoechst staining at the senescence plateau.
The presence of annexin-FITC+/PI− cell populations following HY-PDT treatment, in addition to cellular and nuclear damages as evident from DNA fragmentation experiments, and the formation of ROS are suggestive of recruitment of mechanisms consistent to apoptosis.
Two different methods were employed as described previously; FCM analysis of DNA integrity, by which apoptosis is represented as the sub-G1 population (Iwadate et al, 1997, 2002), and morphological observation of nuclear damages (Sekiya et al, 1991).
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The literature data for nuclear damage regime have been collected and critically examined.
After irradiation at low fluence the marker profiles follow the nuclear damage distribution even in three dimensions.
The polymer's penetrant uptake can be described well by regular diffusion, with nuclear damage centres acting as saturable traps.
The stages of apoptosis were evaluated by AO/EB staining and nuclear damage was determined by Hoechst 33258 staining.
"The movie is now locked except for the soundtrack, but just today we added an explanatory line for a refrigerator question about nuclear damage to the earth.
Liability and compensation for nuclear damage is covered under this Act up to a current limit of £140M for 10 years after the incident.
The presence of severe nuclear damage, revealed cells progressing towards abnormal genotypes, due to a series of aberrant mitosis followed by unequal distribution of chromosomal content.
18β-Glycyrrhetinic acid induced the nuclear damage, changes in the mitochondrial membrane permeability, formation of reactive oxygen species and depletion of glutathione in SiHa cells.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com