Some readers, mindful of the faulty intelligence and reporting about Saddam Hussein's weapons program, are watching the Iran nuclear coverage very closely.
Following assembly, the GSR dataset represents a nuclear coverage of ~78 Mb, which is equivalent to 52% of the sampled gene space and 12.7% of the total cowpea genome.
In addition, DNA samples were extracted from muscle tissue of 10 unrelated wolverine individuals (all males from different parts of the Scandinavian population) and were sequenced to approximately 10X nuclear coverage each.
Using avian genome sequencing data from a single collared flycatcher with a nuclear genome coverage of 85X, we analysed variation in coverage of mtDNA in a similar way as above.
After aligning the sequences to the draft 'Lovell' genome which contains only nuclear DNA, coverage depth for DD, F8, and GB were calculated to be 15.64×, 12.56×, and 13.29×, respectively, for a total coverage of 41.5× of the nuclear genome.
Interestingly the wAnaITG genomes examined in this study had on average twice the D. ananassae nuclear genome coverage (table 1), whereas Klasson et al. (2014) reported the wAnaITG samples from India and Indonesia to have far less coverage than the D. ananassae nuclear genome.
(A ) Mean nuclear genome coverage from historic (red) and modern (blue) samples.
This level of sequencing corresponds to approximately 7.5× expected average nuclear genomic coverage using 2C values from Godsoe et al. (2013).
For 454 sequences sets of 813,770, 814,668, 771,864, 704,918 and 692,688 shotgun reads corresponding to an estimated nuclear genome coverage of 0.6×/set were used for initial assembly and 570,590 3 kb paired-end reads were used for connections verification.
For Illumina sequences, out of 51 million reads (corresponding to a 10× nuclear genome coverage), 6,949,870 sequences were successfully mapped to the plastid genome and 1,365,825 sequences were mapped to the mitochondria giving a 4,385x and 452x coverage, respectively (Additional file 3: Table S1).
Using the median read depth as an estimate of the number of rDNA copies sequenced and the nuclear genome coverage of approximately 0.4×, rough approximations of the number of 5S rDNA repeats and of rDNA cistron copies are 1,015 and 1,845 respectively.
