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Nuclear area (μm), perimeter (μm) and cytoplasm area (μm) were measured and the form factor (4π X nuclear area / [nuclear perimeter]) was calculated.
Yellow triangles: nucleus part of the great nuclear area.
Green triangles: non-nucleus part of the great nuclear area.
Specifically, the projected nuclear area increased and cartilaginous gene expressions decreased during in vitro expansion culture.
For the cytometric analysis, nuclear area, proliferation index, and ploidy within random fields were estimated in Feulgen's stained sections.
nuclear area 2).
This result strongly suggested that the ACTN4 is essential for maintaining normal cellular and nuclear area and that both ACTN1 and ACTN4 are able to contribute to the maintenance of cellular and nuclear area.
The DAPI image was used to define the nuclear area and the Cy3 image to quantify of telomere fluorescence.
The nuclear area was defined using NUP153 as nucleus boundary.
Also, R11K did increase the average nuclear area.
In the case of Ki67, the ratio of positive nuclear area to total nuclear area was employed.
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