Sentence examples for nub protein from inspiring English sources

Importantly, Nub protein did not bind to the Act5C or to a non-transcribed intergenic region.

Except for distinct localization in midgut enterocytes [ 29], it is not known if Nub protein is expressed in immunoresponsive tissues.

Nub protein bound to the promoter regions of CecA1, CecC, AttC and DiptA were isolated using the Nub-specific antibody.

To analyze if Nub protein directly binds to the promoter regions of AMP genes, we carried out chromatin immunoprecipitation assays (ChIP).

Immunostaining of adult tissues in cryosections of whole flies revealed that Nub protein is present in fat body, midgut and testis (unpublished).

Strong immunostaining in wing and leg imaginal discs of third instar larvae confirmed localization of Nub protein in these tissues (Additional file 2C and unpublished).

Thus, in the absence of a functional Nub-PD protein as in nub 1 mutants, the gut microbiome is severely affected, most likely as a consequence of the increased expression of AMPs and possibly of other immune effector molecules.

Nub-PD protein binds to upstream sequences and represses gene expression of several AMP genes in healthy flies.

Importantly, flies that lack expression of Nub-PD protein have a significantly changed gut microbiome, indicating that the maintenance of a normal gut flora is dependent on negative gene regulation by Nub-PD.

One of these, corresponding to the nub-RD transcript, activated expression of a Cecropin A1-luciferase (CecA1-luc) reporter in Drosophila cells, indicating that the Nub-PD protein is able to bind and regulate transcription from the CecA1 promoter.

The availability of genomic sequences from other Diptera, including 24 mosquito species, the Mediterranean fruit fly (Ceratitis capitata or medfly) and the housefly (Musca domestica), has allowed us to compare the Nub and Pdm-2 proteins in each of these species and determined their sequence relationship (Fig.  1b).