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The identification of disc enhancers is in agreement with previous studies that have detected Nub expression in wing discs via immunostaining [ 25, 33].
For example, previous studies have shown that nub expression is relatively high in multiple tissues during embryogenesis and is steadily reduced in the larvae and adult, whereas pdm-2 transcripts are detectable during embryonic and larval development [ 10– 10].
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Using a heat-shock promoter-driven Gal4 (UAS-nub-RD/hs-Gal4) it was also not possible to achieve high levels of nub mRNA expression after heat-shock induction, although several different experimental regimes were tested.
In agreement with the transient overlap of Nub and Cas expression [ 3], co-localization of nub-46 enhancer activity and Cas in NBs was observed during late developmental time points (Fig. 6b– d).
A) nub-8 regulates expression in a subset of anterior and posterior ventral nerve cord (VNC) neurons B) nub-9 drives expression mostly in a subset of lateral VNC neurons.
E) nub-12 drives expression in a subset of posterolateral VNC neurons.
F) nub-13 regulates expression in a subset of posterolateral VNC neurons.
C) nub-10 regulates expression in a subset of lateral VNC neurons.
D) nub-11 directs expression in a subset of anterolateral VNC and central brain neurons.
For example, nub-29 regulated expression in both the optic lobe and central brain tracheal branches (Fig. 9b).
Of the five disc enhancers, nub-31 drove expression in a subset of cells occupying the dorsal anterior region of the leg imaginal disc [ 34] (Fig. 7f).
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