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NTG induces CGRP release from NTC for a period of 4 h after its administration, while formalin injection induces an ipsilateral decrease in CGRP in the NTC 1 h later [29, 30].
Signal-to-noise ratios (SN) were calculated by dividing the MFI of the sample by the corresponding MFI of the NTC (1).
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The nestedness measures used were NODF [ 26], NTC [ 27, 28] and BR, the discrepancy score of Brualdi and Sanderson, 1999 [ 29].
CB1 receptors are localized on fibers in the spinal trigeminal tract and in the NTC [6].
The example protocols include Local Information No Topology LINT /Local Information Link-statee TopoLILT (LILT) [8], MobileGRID [9], Novel Topology Control Protocol (NTC) [10], and Cooperative Nearest Neighbor (CNN) [11].
The bilinear curve gives a behavior factor that is defined as the ratio between the maximum displacement and the yielding displacement equal to 1.34, lower than the value suggested by the Italian code (NTC 08).
NTG may induce indeed a direct hyperalgesic effect via the formation of nitric oxide (NO) and via CGRP release in the NTC [42, 48], or indirectly via the activation of NOS synthesis at the meningeal level as a consequence of a sensitization of the trigeminovascular system [49 51].
Our results are in agreement with data obtained in another animal model of migraine, where it was shown that activation of CB1 receptor reduces Fos immunoreactivity induced after activation of the ophthalmic division of the trigeminal nerve, in neurons of the NTC [37].
The behavior factor has been assumed equal to 2.25 that is the value suggested by the Italian building code (NTC 08) for towers irregular in elevation like in the case here considered, which is characterized by the presence of the Cathedral only on one side of the tower.
Additionally, final raw fluorescent signals (RFUs) generated by PCR with ILPs were significantly stronger than those generated using the random primers (1,200,000 RFUs vs. 320,000 RFUs, data not shown), and both were much higher than the negative control (NTC, 37,000 RFUs; data not shown).
Christie-Atkins-Munch –Peterson (CAMP) testing was performed on colonies accompanied by a positive control (NTC 3110, NTC 8840).
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