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where Nt 1(s i ) and Nt 1(s j ) represent the number of transmission for data generated by s i and s j before arriving at g u (x0, y0).
TbUNC119 cDNA (nt 1 to 468) was amplified by PCR using primers, 5'-CTCGAGCCATTCTGCATTGAATTGCG-3' and 5'-AAGCTTATGGCTGCGACGACCGAGGTC-3', which contained restriction sites for XhoI and HindIII, respectively.
A Pf-5 gacA mutant (JL4577) was constructed previously by replacing 626 bp (nt 1 to 626) internal to gacA with aphI, which confers kanamycin resistance [16].
The coding sequence (16 amino acids) of ORF5 is between nt 52 and nt 1.
One is located on chromosome 2 (nt 1 767 933 to nt 1 772 818, referred to hereafter as Osj2) and the other on chromosome 6 (nt 25 706 265 to nt 25 701 456, referred to hereafter as Osj1).
†The amplicon generated with these primer spans across nt 1 of the 5,229-bp 5,229-bp virus genome.
Similar(53)
Two adjacent promoters P1 [−2521 nucleotide (nt)/−597 nt] and P2 (−2521 nt/+1 nt) initiate transcription of alternative first exons (U1 and U2).
MicroRNAs (miRNAs) are small non-coding RNA sequences of about 22 nt [1].
MicroRNAs (miRNAs) are recently identified endogenous small non-coding regulatory RNAs (∼22 nt) [1], [2].
Blots were hybridized with the ChpPV NS, Cap and NSCap probes, which were amplified ChpPV DNA fragments of nt 1 1561, nt 3281 5205 and nt 1 5205, respectively, from the pC1ChpPV.
Seeds (nt 1 8 and 2 9) of all 470 human miRNAs listed in the Sanger miRNA Registry (http://microrna.sanger.ac.uk, version 10.0), were selected.
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