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Sequences containing Ns were removed as per the software requirements.
For the alignment-free analysis, padding Ns were removed.
Additionally leading and trailing Ns were removed as well as bases with quality 2 or less.
Again, Ns were removed from the sequences of up to 23,004 contigs.
Following this, windows that covered a segment of the assembly with greater than 50 % Ns were removed.
Genomic segments containing runs of five or more Ns were removed, as were transcripts with a MAKER-derived AED score > 0.2 (indicative of transcripts with lesser evidential support).
Similar(53)
However, only 34.04% of the TOC and 36.99% of T-N were removed under optimal concentrations, indicating formation of intermediate products during CBZ degradation.
While on average 85% of K and 69% of N were removed from the particulate fraction, only around 20% of Mg and 10% of P were dissolved in the process waters.
Regions of ambiguous sequence (N) were removed from the analysis.
Sequence reads that contained undetermined bases ("N") were removed.
All sequences with at least one 'N' were removed.
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