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An additional 6 ns were collected and used to confirm the results of the longer NPT ensemble simulations.
For differentiation, 7 day-old NS were collected into 50-ml tubes and centrifuged for 3 minutes at 100xg.
Samples of NS were collected from a remainder of non sun-exposed skin, which was used as donor skin for performing a skin graft.
The conditioned media from CT26 cells infected with rAAV2-hPEDF, rAAV2-null, or NS were collected as described above, and each supernatant was two-fold serially diluted and added to each well in triplicate.
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Supernatants containing soluble nucleic proteins (N) were collected by centrifugation at 12000 g for 10 min.
Nuclei from each ploidy distribution (1.7 N, 2 N, 3.1 N, and 3.3 N) were collected in separate 1.5 mL tubes and DNA was isolated and purified using the Qiagen QiaAMP mini kit (cat #51304).
The samples were collected at 8 30 AM to 4 30 PM during the period of the experiment and physiochemical parameters of temperature, color, pH, DO, COD, TN, TP, and NH3-N were collected on the specific monitoring points of both rivers.
After 10 min, the white (HSiO1.5) n was collected by centrifugation, washed by distilled water, and dried in vacuum at 60°C.
Torque (Nmm), rotation, time (s), and load (N) was collected using MTS software.
Sand samples (n =17) were collected from sand piles that contained cat feces; a soil sample (n =1) was collected from a square to which the feral cats had common access at night; bark samples (n =5) were collected from trees with signs of feline scratches near a known home of diseased cats; a sample of decaying wood (n =1) was collected for mycological investigation.
Nasopharyngeal swabs (n 1120) were collected from outpatients between 2004 and 2009 within an ongoing nationwide surveillance program.
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