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The meeting rate due to the movement of species A is given by khop,A ns(A, t)/ Nsites.
After a sufficiently long time (here 15 ns), a steady-state condition is reached.
The TDC system operates with an input resolution of 1.2 ns, a minimum input pulse width of 4.8 ns and a minimum separation of 4.8 ns between pulses.
The response time of wavelength switching of <6.8 ns, a 3.38 nm tuning range, and a side-mode suppression ratio of >23 dB have been achieved experimentally.
Finally, by passing through the central G-tetrad, the K+ reached the lower binding site at 187.60 ns, a conformation achieved till the end of observation (Fig. 3F).
After 1.7 ns a small red shift is observed because of the disappearence of feature |1〉, while after 12 ns peak |3〉 becomes dominant.
Similar(19)
For oligodendroglial differentiation cells were plated on polyornithin/laminin-coated dishes, and the NS cell expansion medium, which is composed of NS-A medium (Euroclone, Pero, Italy) plus N2 supplement (Invitrogen; Karlsruhe, Germany), was replaced by DMEM/F12 supplemented with N2 (N2 medium).
After 72 hrs, cultures were shifted to NS-A control medium (CM) containing 2% fetal calf serum (FCS) and grown for two weeks.
After 72 hrs, cultures were shifted to serum-deprived NS-A control medium (CM) and grown for up to two weeks to obtain a mixed culture of neural cells containing astrocytes, neurons and oligodendrocytes [26].
TICs were cultured in NS-A basal serum-free medium (Euroclone) containing 2 mM L-glutamine, 0.6% glucose, 9.6 µg/ml putrescine, 6.3 ng/ml progesterone, 5.2 ng/ml sodium selenite, 0.025 mg/ml insulin, 0.1 mg/ml transferrin sodium salt, supplemented with 20 ng/ml EGF and 10 ng/ml basic FGF.
E18 ESSCs were cultured using the NeuroCult NS-A ProliferandoNeuroCult NeuroCult NS-A Differentiation Kit (rat) from Stem Cell Technologies, for the induction of proliferation and differentiation, respectively.
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