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HMS media from NP cells contained 134.7 ± 15.2 pg/ml TNFα compared to 112.3 ± 6.03 pg/ml in the static NP media (P = 0.0504), which approached statistical significance.
HMS NP cultures contained 5.64 ± 2.7 pg/ml NGF compared to 3.91 ± 2.01 pg/ml βNGF in the static NP media.
The plant cells accumulated ROS in CuO NP media partly because CuO NPs released Cu2+ in the culture media.
The plant accumulated more reactive oxygen species in the CuO NP media than in the same concentration of bulk CuO.
Western blot analysis of CP vs. NP media confirmed overexpression of AGR2 protein in cancer.
Quantification of neurite outgrowth revealed that 78.9% ± 1.8% of cells (P = 0.00039 vs. control) contained neurite extensions after treatment with NGF, whereas culture of cells with both HMS AF and NP media resulted in 39.4% ± 2.4% and 42.6% ± 3.8% of cells with neurites, respectively (P = 0.00078 and P = 0.0039 vs. control, respectively).
Similar(54)
L. minor roots were easily broken in CuO NPs media under the experimental condition, and the inhibition occurred only partly because CuO NPs released Cu2+ in the culture media.
The plants in CuO NPs media accumulated O2 − in comparatively low concentration, and the release of Cu2+in culture media acts as a very important factor in SOD activity increase.
The POD, SOD, and CAT activities of L. minor increased when the plants were exposed to CuO NPs, bulk CuO NPs and two times the concentration of Cu2+ released from CuO NPs in culture media, but the increase of these enzymes were the highest in CuO NPs media among the three kinds of materials.
The observed lower Zn2+ dissolution of the nZnO – FBS/PBS sample may be due to the presence of serum proteins from FBS on the nZnO surface, reducing direct NP-media interaction and thus protecting them from Zn2+ dissolution.
The insignificant decrease in L. minor chlorophyll content in low CuO NP concentration media indicated that the oxygen and glucose synthesis proceeds normally in these culture conditions.
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