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Different NOX preparations (soluble and immobilized) were incubated in 50 mM sodium phosphate at pH 7 and 83°C.
The activities of the different NOX preparations were analyzed by following the decrease in absorbance at 340 nm corresponding to the oxidation of NADH.
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Immobilization on Gx-ag matrixes yielded the most stable NOX preparation.
The activities of soluble and CNBr-NOX preparations were assayed at different temperatures (from 25 to 90°C) in 50 mM sodium phosphate and at different pH values (pH 5-10).
Our experiments to measure exclusively NOS-derived NOx in activated platelet preparations indicated that the intermediate concentration of NAC (30 μmol/l) increased NOS-dependent NOx formation by ~2.5-fold.
In recent years, the risk of anhydrous and aqueous ammonia in transportation and storage has been a concern of power plants around the world, and the urea hydrolysis to ammonia using low-grade steam as heat source is gradually used for feedstock preparation in NOx reduction.
For these purpose, we performed standard NOX assays using either a preparation released at pH 5 from HeLa cells obtained from Cellex Biosciences (Minneapolis, USA), which is a mixture of NOXs (Del Castillo-Olivaret et al, 1998), or from intact HeLa cells.
All the fresh catalysts show high activity toward the selective catalytic reduction of NOx with NH3 (NH3-SCR) at low temperatures regardless of preparation method.
Immobilization of NOX onto agarose activated with glyoxyl groups yielded the most stable enzyme preparation (6-fold more stable than the hyperactivated soluble enzyme).
Mechanochemical preparation of Ag/Al2O3 catalysts used for the selective catalytic reduction of NOx using hydrocarbons has been shown to substantially increase the activity of the catalyst in comparison with Ag/Al2O3 prepared by wet impregnation.
a 6BCL agarose was activated with different functional groups as methods described in order to immobilized NOX through different chemistries, giving each one different properties to the final insoluble preparation.
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