Three novel sites were identified.
Further studies showed that Cdk1 directly phosphorylates TAZ on six novel sites (serine 90, serine 105, threonine 175, threonine 285, threonine 326, and threonine 346).
In conclusion, our computer search identified some known HNF1 sites, and suggested three novel sites indicating these genes are very likely to be directly activated by HNF1.
A single amino acid change at the I785 cleavage site to proline resulted in partial MMP-1 resistance, but cuts were found in novel sites in the original cleavage region.
However, we believe that by training a classifier to predict ligandability of pocket points (that represent local chemical neighborhood rather than the whole pocket) we provided a way for sufficient generalization and therefore ability to correctly predict ligandability of novel sites.
Mass spectrometric analysis reveals several sites of modification that comprise previously identified as well as novel sites.
The transcripts initiating at these novel sites are differentially expressed with the shorter isoform 4 (PCA3-4) more highly expressed in PCa and metastases.
Table 1 contains a category-wise count of conserved pY events that are confirmed in vivo sites in human proteins as well the novel sites that are not reported so far.
Furthermore, these results demonstrate that despite the numerous rounds of previous MS analysis, additional series of MS analyses employing recent technological advancements are necessary for continued identification of novel sites of modifications to generate a more complete atlas of the factors that putatively function in the context of the histone code.
MHV-68 was used to model infection of the lymphoid system enabling fundamental questions to be addressed on the kinetics of latent infection, the detection of novel sites of latency and the potential to explore host and virus gene expression in individual infected cells at different stages following infection.
Importantly, the current study indentifies novel sites of mutation in the exon 4 of βI-tubulin that are able to induce both drug resistance to microtubule de-stabilizers and hyper-sensitivity to microtubule-stabilizers through both alterations in the drug-binding site and microtubule stability.
