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While this appears to be an excellent hypothesis, knockdown of the DEPDC2 gene along with CPA6 in the zebrafish, using specific splice-blocking morpholinos, did not result in any noticeable defects in eye abduction (data not shown).
The AMD-r gene is expressed in tissues that produce cuticle and noticeable defects in regions of flexible cuticular structures have been observed in AMD-r gene mutants [14] [16], but the biochemical mechanism/pathways involved in flexible cuticle formation are unknown.
IME2/ime2-DFGx strains had no noticeable defects in meiosis or sporulation.
In contrast, individual loss of the β3or β5 subunit during embryogenesis does not cause noticeable defects in the formation of the cardiovascular system (Hodivala-Dilke et al, 1999; Huang et al, 2000).
Thus, our results are not in conflict with the finding that mice with complete deletion of CD13 have no noticeable defects in Fc γR- or scavenger receptor-mediated phagocytosis [ 14].
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Cell cycle analysis by flow cytometry showed that fun30-deletion mutants do not exhibit a noticeable defect in cell cycle progression (Figure 3 B, wt, fun30Δ).
Unlike in ctf18Δ cells, we could not observe any noticeable defect in sister chromatid cohesion in fen1Δ and dna2-2 cells.
Initial studies strongly suggested that occludin was not required for the TJs formation or the maintenance of barrier function as occludin knockout mice lacked any noticeable defect in intestinal TJs morphology or barrier function [ 36].
However, embryos shifted at later stages exhibited a progressively reduced loss of pPI cells the later the shift occurred; at stage 17, there was no noticeable defect in the size of the pPI (0/5 cases, as with the control; Fig. 4C).
Combined loss of BOK and BAK did not elicit any noticeable defects, although it remains possible that BOK and BAK have critical roles in developmental cell death that overlap with those of BAX.
Both the hot embossing and UV nanoimprint patterning processes were done on large size substrates without any noticeable defects.
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