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Both the hot embossing and UV nanoimprint patterning processes were done on large size substrates without any noticeable defects.
While this appears to be an excellent hypothesis, knockdown of the DEPDC2 gene along with CPA6 in the zebrafish, using specific splice-blocking morpholinos, did not result in any noticeable defects in eye abduction (data not shown).
The AMD-r gene is expressed in tissues that produce cuticle and noticeable defects in regions of flexible cuticular structures have been observed in AMD-r gene mutants [14] [16], but the biochemical mechanism/pathways involved in flexible cuticle formation are unknown.
IME2/ime2-DFGx strains had no noticeable defects in meiosis or sporulation.
Combined loss of BOK and BAK did not elicit any noticeable defects, although it remains possible that BOK and BAK have critical roles in developmental cell death that overlap with those of BAX.
In contrast, individual loss of the β3or β5 subunit during embryogenesis does not cause noticeable defects in the formation of the cardiovascular system (Hodivala-Dilke et al, 1999; Huang et al, 2000).
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Cell cycle analysis by flow cytometry showed that fun30-deletion mutants do not exhibit a noticeable defect in cell cycle progression (Figure 3 B, wt, fun30Δ).
There were no wing hair defects (orientation or number) or any other noticeable defect associated with the loss of dGIPC either in homozygous animals (even when the maternal contribution was removed), or in homozygous somatic clones.
Unlike in ctf18Δ cells, we could not observe any noticeable defect in sister chromatid cohesion in fen1Δ and dna2-2 cells.
The first noticeable defect was observed at telophase I when lagging chromosomes were observed, followed by mis-segregated chromosomes at prophase II.
The first noticeable defect was the appearance of lagging and broken chromosomes during telophase I. Twenty or more individual chromosomes were often observed beginning at metaphase II, suggesting that cohesion might be prematurely lost.
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