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For human trisomic cells, we also observed the emergence of euploid cells without noticeable cell death in culture.
For mouse ES cells, we observed that karyotype improvement occurred within a few days without noticeable cell death.
On day 35, rACs remained round and viable in all cultures, except Ind-P1rAC showing slightly noticeable cell death, 2(c), 2 e), and 2(f)).
Notably, the colony formation data indicate that a 4 h treatment with 3 μM Mito-ChM was sufficient to induce significant anti-proliferative effects in both MCF-7 and MDA-MB-231 cells without noticeable cell death under those conditions.
Similar to our previous study [ 14], PCV2 was easily internalized in the cytoplasm of AMs but caused no noticeable cell death; and PRRSV displayed a low infectious rate but severe cytopathic effect and strong TNF-α induction in AMs.
In our study, we found that (i) RA is able to sensitize breast cancer cells to the anti-proliferative effects of poly(I C) and (ii) RA does not induce a noticeable cell death in breast cancer cells by itself but it synergizes with poly(I C) to increase cell death.
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A higher percentage of apoptotic cell death is noticeable in cells cotreated with TNF α and PD98059, and the level of apoptotic cell death reached is similar to the one observed in cells stably transfected with SR-I κB α and treated with TNF α alone.
It is noticeable that photothermal therapy-induced tumor cell death provided a source of tumor antigens released from the host's own tumor.
Cresyl violet staining confirmed that this decrease was not due to cell death as no noticeable change in cell density in the ischemic area was observed between VEH- and PUR-treated groups at this time point (Supplementary Figures S1H and J).
By 24 h post-infection, there was evidence of ethidium homodimer incorporation (an indicator of cell death) (Fig. 2E) and a noticeable reduction in overall cell number (Fig. 2F) in adFOXO3a infected cultures.
Unlike with S. typhimurium, rough (but not smooth) B. abortus-induced macrophage cell death exhibits caspase-2 activation and is noticeable hampered by a caspase-2 inhibitor and caspase-2-specific shRNA (Figure 6 and 9).
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