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Unless noted, reagents were purchased from Sigma.
Unless otherwise noted, reagents and organic solvents were obtained from commercially available sources and used without further purification.
The cells were washed and then cultured in complete media containing the noted reagents and inhibitors.
Cultured VSMCs were plated on cover slides in six-well plates and treated with noted reagents for 24 h in serum-free DMEM.
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Barcoding will note reagents, samples, and instruments used, providing context to the data for subsequent analysis.
Except as noted, other reagents were of either reagent grade or molecular biological grade from Sigma.
Unless otherwise noted, all reagents were purchased from Sigma-Aldrich (St . Louis MO) and used without further purification.
Unless otherwise noted, all reagents were purchased from Thermo-Fisher.
Unless otherwise noted, all reagents, including anhydrous solvents, were obtained from Sigma-Aldrich (Poole, UK).
After transfer to a polyvinylidine difluoride membrane, a primary antibody (as noted in "Reagents") was added.
Unless specifically noted otherwise, reagents were purchased from Sigma-Aldrich (St . Louis MO), and enzymes were purchased from New England Biolabs (Ipswich, MA) or Roche Life Science (Indianapolis, IN).
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