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Rice transformation is as described previously [13], [14] with minor modifications noted in Methods S1.
Q-PCR was performed as described previously [19] with modifications noted in Methods S1 and using primers in Table S3.
As briefly noted in Methods, the subtraction of the mean CW from each CW leaves subtraction artifacts in the steepest phases of the CW, often including the fast positive and negative portions of the postsynaptic AP (see Figure 5A2/B2).
Thus we used multivariate analysis to control for the covariates noted in "Methods".
(P. simiovale was grouped into the P. fieldi lineage in this study, as noted in Methods).
These ranges slightly exceed the primer-design guidelines noted in Methods.
Similar(46)
As noted in " Method", only the significant cross paths related to the study's examination of the effect models are presented.
As noted in the Methods section, the magnitude (although not the direction) of the reported results could be contingent on the method used to calculate the scores.
As noted in the methods section, we observed that the rates did not necessarily remain constant beyond approximately 20 minutes.
Adequate suctioning was obtained in both groups and there was no other significant complication related to the suctioning noted in both methods.
As noted in the Methods, these were truncated accordingly and filled to 64 bases with polyA.
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