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Unless otherwise noted, experiments were conducted at 37°C.
Unless otherwise noted, experiments were performed in young adult mice, 8 12 weeks in age at the outset of any treatment.
Also unless otherwise noted, experiments were performed in DM25 medium, in which glucose at 25 μg/ml is the only usable source of carbon.
Except where otherwise noted, experiments were performed in a working buffer containing 100 mM Tris-HCl (pH 7.6), 10 mM NaCl, 0.1 mM EDTA.
Unless otherwise noted, experiments were performed in a 15 μL volume in 20 mM Tris-HCl, 125 mM NaCl, 6 mM MgCl2, 1.3 mM ATP, 0.2 mg/mL BSA, 2% glycerol, pH 8.0.
As noted, experiments were started with ES plates designed as a diagnostic tool to distinguish between E. coli and Salmonella sp. When PM1 and PM3 plates became available, we chose 21 archival strains from the ES list to score for differences in catabolism of 190 different substrates with Biolog™ PM1 and PM3 plates.
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We noted experiment-to-experiment variability in survival after drug treatment (for example, compare the numbers for wild type in panels A and B of Fig. 2).
All experiments used this food protocol unless otherwise noted (specifically experiments using instant Drosophila media with RU486 experiments).
It is noted that experiments are time consuming and cost expensive.
Update: This post originally noted an "Experiments" section was available under "Settings".
Unless otherwise noted, all experiments were done three times.
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