Of note, experiments performed in primary cortical neurons indicated a significant protection in bmf-deficient neurons both in vitro after OGD- and NMDA-induced excitotoxic injury.
Of note, experiments performed with incubation of TNT003 with HAEC + HLA-Ab, prior to addition of NHS, did not significantly impair TNT003 blockade of C4d deposition (data not shown) suggesting no temporal restrictions on TNT003 function.
Notes Experiment 1 assumes a fixed TFR around 5 and a life expectancy at birth of 31.5 years.
Whether that means downloading an app that helps you organize your day, writing down a list on a piece of paper, or even using sticky notes, experiment with different systems and stick to the one that works best for you.
Unless otherwise noted, experiments were conducted at 37°C.
Unless otherwise noted, experiments were performed in young adult mice, 8 12 weeks in age at the outset of any treatment.
We noted experiment-to-experiment variability in survival after drug treatment (for example, compare the numbers for wild type in panels A and B of Fig. 2).
Also unless otherwise noted, experiments were performed in DM25 medium, in which glucose at 25 μg/ml is the only usable source of carbon.
Except where otherwise noted, experiments were performed in a working buffer containing 100 mM Tris-HCl (pH 7.6), 10 mM NaCl, 0.1 mM EDTA.
Unless otherwise noted, experiments were performed in a 15 μL volume in 20 mM Tris-HCl, 125 mM NaCl, 6 mM MgCl2, 1.3 mM ATP, 0.2 mg/mL BSA, 2% glycerol, pH 8.0.
Note that Experiment 1b and Experiment 4c are, in a way, opposites.
