But be forewarned: The courts of England and Wales are warming to the idea of enforcing prenups, even if they are not yet strictly binding under law.
Collectively, these studies establish a direct thermodynamic linkage between inhibitory helix stability and ETV6 autoinhibition, and demonstrate that helix unfolding does not strictly precede DNA binding.
The binding of the μ2PIP-Core mutant to PtdIns4,5P2/[ED]xxxL[LI] liposomes is only reduced by 3-fold, suggesting that acidic dileucine motif binding does not strictly need C-μ2 to bind to the membrane, only its displacement from the bowl.
Potencies were not strictly related to receptor binding affinities and not significantly affected by the amount of endogenous GC receptor.
This suggests that Asp, and perhaps other conserved SMN residues in this region that are not strictly required for Gemin2 binding, may be involved in higher-order interactions within the oligomeric SMN complex [ 26].
Although the kinetics of C/EBPα binding did not strictly match the expression profile of hepcidin, our fEMSA results suggest that modulation of C/EBPα binding to hepcidin promoter might underlie the up-regulation of its expression detected after 24 h of DTT treatment.
Interestingly, repression of the FLC floral transcriptional regulator in lif2 mutant is accompanied by an increase in H3K27 trimethylation at the locus, without any change in LHP1 binding, suggesting that LHP1 is targeted independently from LIF2 and that LHP1 binding does not strictly correlate with gene expression.
Also, the evolution of binding sites is not strictly qualitative: a site may be present, but with a changed binding rate.
The increase in splicing observed with ΔRS harboring the R8E mutation (Figure 1C, lane 19) was not strictly correlated with improvement in RNA binding as measured by UV crosslinking with purified recombinant proteins, as apparent binding of ΔRS: R8E protein to IgM M1-M2 RNA was not greatly increased relative to binding of ΔRS (Figure 3B, lanes 4 and 7).
The work indicated that activation of the ataxia-telangiectasia mutated (ATM) protein - a crucial player in DNA damage recognition - potentially results from structural changes of chromatin organization and may not strictly depend on direct protein-DNA binding [57].
