In some instances, murine models of allergic asthma have not recapitulated the human findings, and have led to some costly failures in drug development.
However, these observations are not recapitulated on application of transmural pressure (PTM) oscillations (that mimic tidal breathing and DIs) in isolated intact airways.
The allogeneic extracellular matrix derived hydrogels elicited significantly reduced total, human specific, and CD4+ T-helper cell infiltration in humanized mice compared to xenogeneic extracellular matrix hydrogels, which was not recapitulated in wild type mice.
The ropB mutation of strain 5628 was not recapitulated on passage in the mouse subcutaneous infection model.
In addition to the usual caveats of overexpression data, this result was not recapitulated by the same group when using a different plasmid system [22].
However, this seems unlikely because such an effect is not recapitulated in our growth curves (Fig. 8C and 8D, Figure S5).
The enhanced sensitivity to low-dose STZ was not recapitulated in WT mice denuded of intestinal flora upon the long-term administration of broad-spectrum antibiotics.
Since the characteristic DRBP76 isoform distribution in normal tissues is not recapitulated in transformed cells, this left us with performing analyses of the ∼90 kDa form characteristically associated with CNS malignancy or transformed cells.
Both MRC5 and U2OS cell lines were subsequently used in parallel for most experiments to ascertain if any observed effects required the more reliable cell cycle checkpoints of primary cells (MRC5) and thus were not recapitulated in transformed cell lines such as the U2OS line that has stable p53- and pRb-dependent checkpoints but is defective for p16INK [24], [24].
Although the diffusion-limiting properties of cartilage are not recapitulated in cultured chondrocytes, the absence of active rhGALNS and subsequent accumulation of KS, as well as expression of chondrogenic genes, make MPS IVA chondrocytes a valuable new model of human MPS IVA in vitro.
Again, this is not recapitulated in the ChIP-qPCR.
