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Mosquitofish exposed to PME in the Fenholloway River were generally smaller in length and mass, anal fin segment number was greater, and the number of embryos, but not oocytes, was significantly decreased compared to the reference site fish.
Use of donated semen, but not oocytes, is allowed.
mei-1 mRNA was not identified (File S1), likely because this mRNA is a target of OMA-1-mediated repression in embryos, but not oocytes (Li et al. 2009), which was the basis for our purification.
We also found that vit mRNA levels were low in a feminized mutant unable to produce sperm, but were normal in a masculinized mutant that produces sperm but not oocytes.
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Jiang, L. et al. Sperm, but not oocyte, DNA methylome is inherited by zebrafish early embryos.
Our results showed that GVBD in cumulus-enclosed oocytes (CEOs) but not in denuded oocytes (DOs) was induced by EGF in a dose-dependent manner, which indicated that cumulus cells but not oocyte itself were the main target for EGF-induced meiotic resumption.
Thus, vit transcriptional suppression may be correlated with deficits in sperm, but not oocyte, production.
Another study reported that inhibin B levels in the FF are significantly correlated to embryo quality, but not oocyte quality (Change et al., 2002).
Recent reports from zebrafish embryos strengthen the hypothesis that sperm DNA methylome, but not oocyte, is inherited during early embryo formation [ 32].
Consequently, many mRNAs that we either did not screen for or showed not oocyte localization will per definition not be shown in this graph, regardless of their localization mechanism.
In such animals, GFP expression is observed in most, if not all, oocytes and appears to correlate temporally with oocyte formation.
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