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α-Syn not only binds copper but copper binding increases its aggregation [41] [43] and results in the formation of toxic oligomeric species that kill cells both exogenously and endogenously [22], [27].
Moreover, highly accurate DNA-binding site information obtained by GeF-seq enabled us to obtain a comprehensive view of the correlation between AbrB-binding signals and cognate recognition sequences; AbrB not only binds to bipartite motifs in sequences with high binding signals, but also to single-sequence motifs in sequences with low signals.
These results strongly suggested that AbrB not only binds stably, with high experimentally derived binding signals, to bipartite TGGNA motifs, but also interacts with single TGGNA motifs in sequences with lower but significant experimentally derived signal intensities (Supplementary Fig. S4).
This signalling pathway was used to examine whether CHIPS-FITC not only binds FPR1, as demonstrated with the CHIPS-FITC binding assay, but also inhibits the activation by its natural ligand fMLF and mitochondrial peptides fMMYALF and fMLKLIV.
In further analysis of the binding specificity, we found that the MSCRAMM not only binds to the NTD F1 modules [19], [20] and the GBD (manuscript in preparation), but also the 1 2F3, and 3F3 modules.
This data implies that ESTA-1 not only binds to E-selectin, but also may share the same binding site with E-selectin monoclonal antibody used for this experiment.
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Human PrlR does not only bind human prolactin, but is capable of binding and responding to GH, with zinc ions facilitating GH-hPrlR binding [ 44].
In this regard, our recent binding analyses of EMSA showed that MBD1v1 does not only bind to methylated, but also to unmethylated MAGE-A promoters and is able to repress unmethylated MAGE-A promoters [ 11].
Proteasome-shuttling factors, such as the Rad23 proteins, not only bind K48-polyUb conjugates but also, at nanomolar concentrations, stimulate Ub conjugate binding to the proteasome (Nathan et al., 2013).
Moreover, the improvement effect of anti-aging modifier is not only bound up with the aging degree of these bitumens, but also depends on the bitumen nature.
Cardiotoxic drugs not only bind to cardiomyocyte membrane channels to block them but also modify the physicochemical property of membrane lipid bilayers in which channels are embedded.
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