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As expected (see also Fig 3), INr-1 blocked the inhibitory effect of Rev 53 67 but not of Rev 13 23, while INr-2 had the same effect on Rev 13 23 but not on Rev 53 67, again indicating specificity of interaction (Fig 5).
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Tracy Davis told the Guardian in an email: "Mr Jang is not part of Rev Moon's church".
Together, these data indicate that DBC1 is required to maintain Rev-erbα protein levels and that the mechanism involved is not regulation of Rev-erbα gene expression.
Nullbasic alone did not induce CAT expression and downregulation of Rev function was not caused by diminished proviral Rev steady state (data not shown).
However, the effect of DBC1 appears to be independent of GSK3β phosphorylation, since depletion of DBC1 does not prevent phosphorylation of Rev-erbα, and DBC1 can still stabilize a form of Rev-erbα (S55D/S59D) that mimics the phosphorylated state.
The BBC admitted that a new series of Rev will not be made this year and was unable to confirm whether it will be returning in 2013.
Importantly, constitutive expression of Rev M10 did not alter the secretion of interleukin 2 in response to mitogen stimulation of EL-4 and Jurkat cells.
Expression of Rev alone did not lead to induction of cell death by tBid.
The N-terminal 11 residues and C-terminal 7 residues of Rev 1 70 are not visible in the structure.
Unfortunately, it is not possible to unambiguously identify the precise conformation of Rev RRE in the Rev-RRE-Crm1 complex to help produce a more complete molecular model because large parts of Rev and the RRE are not visible in the structure, presumably due to disorder or because they can take on multiple conformations.
As is evident from the results in Fig 1C, INr-1 interacted with Rev 53 67 peptide but not with the Rev 13 23 peptide, while INr-2 interacted with Rev 13 23 but not with Rev 53 67, indicating specificity of interaction.
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