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We clearly showed that NF-κB p65 expressed in endochylema but not in nucleus in normal IEC-6 cells (Fig. 10).
However, in our analysis, Hsp3102-GFP is only localized in the cytoplasm, not in nucleus.
Overexpressed SSB-GFP was observed as a single granule in mitochondrion, not in nucleus in C. merolae cell (data not shown).
In junction tissue, SYL3C-CY3 was expressed in the membrane and cytoplasm but not in nucleus (see row 2 of Figure 3A,B).
In locus coeruleus and rostral ventrolateral medulla, but not in nucleus tractus solitarius, of SHR, noradrenergic and adrenergic vesicles were significantly larger and showed increased NPY colocalization when compared with Wistar rats.
Consistent with other reports, the anti-Bmi-1 antibody used in our study was tumor cell nucleus staining, but contrast to He's report in which Bmi-1 mainly observed in tumor cell cytoplasm less or not in nucleus.
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However in some 4-cell embryos, Sox2 was localized in nuclei in all blastomeres, while in others, nuclear localization was observed in half of the blastomeres, while still in others Sox2 staining was detected only in the cytoplasm and not in nuclei (Figure 1A).
The phenotypes observed in flies are restricted to specific cell types; nuclear envelope detachment phenotypes were observed in imaginal disc nuclei where Lamin C is expressed, but not in nuclei of brains that normally express low levels of Lamin C [30].
On days 15 and 20, immunoreaction was detected in tanycyte cytoplasm, but not in nuclei.
Immunohistochemistry also confirmed striking accumulation of U snRNPs in ALS motor neuron nuclei (Fig 6E), but not in nuclei of hippocampal neurons from FTLD-TDP patients (Supporting Information Fig S5D F).
As shown in Figure2-supplemental Figure 2B, β-galactosidase was detectable in synaptic nuclei, not in extrasynaptic nuclei.
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