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The cross-validation results are very encouraging as a potential biomarker, but we have cross-validated only in colon tissues and not in circulating plasma DNA.
We have addressed both 1a and 1b by expressing RNAi using Hand-gal4, which is expressed in the lymph gland but not in circulating or sessile hemocytes.
Secondly, to check if cluster disruption affects the lymph gland, we made use of DotGAL4, a driver expressed in lymph gland hemocytes but not in circulating and sessile hemocytes (Honti et al., 2010).
During the adrenal dysfunction of CIRCI, impaired cortisol synthesis may also occur upstream of the final 11β-hydroxylase steps, thereby potentially attenuating an increase in 11β-deoxycortisol but not in circulating ACTH via feedback, as during 11β-hydroxylase inhibition [ 3, 18].
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The mRNAs that are often used for analysis of gene expression in tissues (e.g., GAPDH) are not found in circulating blood, presumably because they are not protected by microvesicles/exosomes.
The treatments did not differ in circulating P4 on d −10 or −3, but P4 was greater on d −2 in CHEM cows.
NAgFs and AgFs did not differ in circulating testosterone concentrations, but had significantly lower testosterone levels than did NAgMs.
However, this is not reflected in circulating follicle-stimulating hormone (FSH) concentration, which remains unchanged between control and LCARKOs when examined at d 80 (Fig. 3 G ).
The possibility therefore remains that tumor samples at primary or metastatic sites might demonstrate mutations that do not appear in circulating nucleic acid.
Because clotting has the potential to release particles that are not present in circulating blood, we asked if 5′ tRNA halves circulating in the mouse serum are also present in mouse plasma.
Both DPPIV and NPY are co-expressed not only in circulating immune cells but also in the vascular endothelium, supporting a role for DPPIV in the regulation of systemic NPY [ 75].
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