This transcript is not efficiently, if at all, translated into Atg31 protein, as scanning ribosomes (Kozak 2005) attaching at the 5′ end of the extended transcript would encounter start and stop codons before reaching the ATG31 start codon.
But tomorrow night a dozen of his zany machines will come to life entertainingly -- if not efficiently -- at the Jewish Community Center in Manhattan.
Ste4p, on the other hand, when overexpressed might not efficiently localize at the internal cue.
Thus, the addition of F127 probably does not efficiently adsorb at the dispersed ME particles, and hence a high amount of the stabilizer remains in the excess of water.
In both Dab2-deficient embryos and embryoid bodies, differentiated endoderm cells are not efficiently positioned at the surface, but intermix with epiblast cells.
However, this assumption cannot be validated, because wind shear mechanism is not efficiently working directly at the equator and at very low northern latitudes, where a similar pattern would be expected.
Similarly, although many positive hits from our library screening are known to target PKC, here we show that two selective PKC inhibitors, Go 6976 and Ro 32-0432, do not efficiently kill GSCs at submicromolar concentrations, confirming that inhibition of PKC alone does not induce cell death in GSCs.
In addition to the limitations posed by these other priorities, China's military is moving from 1960s to 1990s technology, and can probably not efficiently absorb technology upgrades at a much faster rate.
It is well established that transfused red blood cells carry but do not efficiently release oxygen for at least 24 hours, because of 2,3-diphospho-glycerate depletion.
In addition, if Ire1α is unable to interact with the channel protein, it does not efficiently cleave mRNA molecules at the endoplasmic reticulum membrane.
Contrary to the promoter-reporter assays which were performed 24 h post-transfection, we could not assay p53 functions for colony formation at 30°C and 25°C in these cells because they did not efficiently attach to the plate at those temperatures for the required amount of time to select with puromycin (7 days).
