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It was also necessary to ensure that the primers did not overlay known single nucleotide polymorphisms (SNPs) that may lead to single alleles not being amplified.
To ensure that the DNA from other bacteria was not being amplified from the swabs and to determine if the mutation was present in one or both of the 23S rRNA genes (rrl1 and rrl2) from T. pallidum, a nested PCR amplification was developed using genes (tp0226 and tp0267) unique to T. pallidum.
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Of these, four could not be amplified and one revealed a mixed infection.
It's terribly exciting singing for so many people, and I've always enjoyed Albert Hall". Miss Sutherland's appearances in London's cavernous Royal Albert Hall have not been amplified.
baileyi, Eimeria sp of dairy cow, Toxoplasma gondii, Eimeria sp of pig, Ascaris suum, Cyclospora sp, and E. coli could not be amplified.
It is also theoretically proved that the queue measurement error or demand estimation error would not be amplified by the QUEUE strategy.
Short DNA could not be amplified efficiently by these methods.
Four of the probes tested could not be amplified, indicating a difference in the ability to detect target transcripts between the heterologous microarray and the PCR platforms.
On the other hand, when the MMSE faces a channel peak the weighting factor will be very small, and therefore the induced interference will not be amplified.
Of the loci that could not be amplified in all isolates, ms217, ms215 and ms77 could not be amplified in 12, 8 and 7 isolates, respectively.
Unfortunately, the complete vacA m region of the Tatshenshini H. pylori strain could not be amplified.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com