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To get a better sense of the extent to which activated subunits can potentiate the activity of holoenzymes that have not been activated, we turned to a solution assay using two different samples of wild-type CaMKII, without fluorescent labeling.
This suggests that activated CaMKII holoenzymes have increased the activity of the holoenzymes that had not been activated previously.
DOI: http://dx.doi.org/10.7554/eLife.01610.014 We also examined the ability of the activated subunits to phosphorylate subunits from holoenzymes that had not been activated.
The first important result is that subunits in constitutively active CaMKIIT286D can exchange with subunits from holoenzymes that have not been activated, when ATP is added.
That mechanism has not been activated by the Ebola diagnosis given to Thomas Eric Duncan, the patient in Dallas.
He said his phone had still not been activated and that he was planning to insist that AT&T honor his existing account.
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They will not be activated.
The system will not be activated to shed heat until next year.
But the suit would not be activated unless its main zipper was closed.
He probably will not be activated until rosters expand on Wednesday.
The automaker said the back-up lights might not be activated when the automatic transmission was positioned in Reverse.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com