Vertebrate centrioles normally propagate through duplication, but in the absence of preexisting centrioles, de novo synthesis can occur.
The induced parasites propagated normally and attained peak cell density at an in vitro concentration of berenil, 5.5-fold higher than the IC100 of the wild-type.
For example, [PSI+] and [URE3] both propagated normally in A5 cells, which grew considerably slower than A3 cells, while in A3 cells [PSI+] was stronger than normal and [URE3] was weaker.
Furthermore, the mOrange negative cells propagated normally in ES medium, forming colonies characteristics of iPS cells, suggesting transient exposure to the bacteria did not alter the iPS cell characters.
Both mutations result in early embryonic lethality, but, as expected from the restricted expression pattern of LRP1b in vivo, loss of Lrp1b does not cause cellular lethality as homozygous Lrp1b-deficient blastocysts can be propagated normally in culture.
Once each clonal cell became a confluent clonal population within the well, they were moved to a larger well with more media until they could be transferred to flasks and propagated normally.
In Jonah Lehrer's previous book, The Decisive Moment, he argued that a psychopath, lacking empathy, suffers from a "broken amygdala" – a part of the brain normally responsible for "propagating aversive emotion".
It's my understanding, however, that if the air molecules are indeed being excited, that actually is producing the pressure waves we know as sound, and those waves will propagate normally, albeit in a less directed fashion.
However, unlike cells grown in the presence of tetracycline, which propagate normally when re-suspended in antibiotic-free media, the DX-producing strain exhibits a delay in its recovery response when re-suspended in LB media devoid of inducer.
We suspect that this defect in cell division leads to a filamentous phenotype in which the cell is no longer able to propagate normally, and this phenotype persists until DX induction is halted and energetic balance is restored to the cell.
