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Results were examined using the SDS 2.2 software, and the relative expression levels of STAT1 were calculated by normalizing with those of GAPDH.
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The recruitment index, R.I., is obtained by normalizing with the subtraction at 'CCDay 0' cell numbers.
The mRNA expression levels were normalized with those of keratin 10 mRNA.
The band intensities of Akt-phospho-protein were normalized with those of total Akt-protein.
mRNA levels of CCT subunits were normalized with those of 18S rRNA in the same samples quantified in the same manner (three replicates).
Daple 40-ct values are normalized with those from CD45, afterwards.
ΔΔCt values were normalized with those obtained from the amplification of the endogenous U6B snRNA (Applied Biosystems).
The copy numbers of each gene were normalized with those of GAPDH.
Finally, the mRNA levels of each transcript were normalized with those of the corresponding CYP2 transcript.
Results of western blotting were normalized with those obtained for β-actin.
ΔΔCt values were normalized with those obtained from the amplification of GAPDH.
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