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Admittedly there are problems in interpreting such data, one of which is the difficulty in normalizing tissue RNA samples for variable epithelial/stromal contribution.
Normalizing tissue D25 levels may provide an adequate substrate for local tissue production of 1,25 hydroxyvitamin D in hematopoietic tissues via extra-renal tissue activity of the 1-alpha-hydroxylase enzyme.
Normalizing tissue 25(OH D levels may provide an adequate substrate for local tissue production of 1,25(OH D in hematopoietic tissues via extra-renal tissue activity of the 1-alpha-hydroxylase enzyme.
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Normalizing DA tissue content to total TH recovered in each region removes the influence of total TH on DA content (Fig. 1c).
Their bacterial content was estimated by measuring luminescence with an FB12 luminometer (Berthold Detection Systems) normalizing by tissue weight.
For bulk samples containing arthropods with a large range of body sizes, a pre-sorting of large samples from smaller ones followed by sub-sampling and re-mixing (tissue normalizing) should help to improve the detection of small individuals, given the total sequencing volume remains at the same level.
Normalizing miR23b expression in tissue lesions, caused by neuropathic pain induction, reduced inflammatory mediators and increased several ROS scavengers.
However, as the soleus contains about twofold more total RNA compared to EDL, normalizing relative to muscle tissue weight showed that MAFbx and MuRF expression were both about twofold higher in the soleus (Fig. 2c).
However, only 2 TEFs are required for normalizing expression in stem tissues.
Since the ratio of an ideal internal control between various tissues would be 1 and the variability of each of our internal normalizing genes between the various tissues assayed was low, we normalized our data to one housekeeping gene, B2M [ 39, 56].
TACs for each tissue were constructed by normalizing decay-corrected time-radioactivity measurements to the standardized uptake value (SUV) of [18F]FDOPA.
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