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The spectra are normalized using a value at 1000 MV.
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The values of QMSP that were obtained from each sample were first normalized using the value of β- actin as an internal reference.
All qRT-PCR reactions were normalized using Ct value corresponding to the actin gene.
In cases where zero instances of a protein family were found in the genomes, GOS data was instead normalized using the value 0.01 (data for all protein families from each step can be found in Additional file 14).
The same was repeated with the Lowess normalized data using a significance value of 0.01.
The left column displays polar maps of averaged radioactivity concentration, normalized using the maximum value of the TCT-AC map.
For each gene's deep sequencing evidence of the 30 samples, each sample was normalized using the RPKM value [ 61].
Each array was normalized using median values of gene expression over the entire array (global normalization).
Data were normalized using Ct values for the house-keeping gene hypoxanthine-guanine phosphoribosyl transferase (HPRT) in each sample.
The hybridization slides were processed by Tiffsplit (Agilent) and data were further analyzed using Genespring software 5.0.2 and normalized using mean values combined with Microsoft Excel software.
The levels of GCS mRNA were semi-quantitated by optical densitometry and normalized using the OD values of glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
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