Sentence examples for normalized to contain from inspiring English sources

Both messenger and total RNA from each of the time points were reverse transcribed under two different conditions; one in which the reactions were normalized to contain equal amounts of RNA and another series of reactions that were not normalized to RNA content.

Each RECQ1 IP (normalized to contain 0.25 mg of protein as the input) was washed twice in lambda phosphatase buffer (New England BioLabs) and resuspended in 50 µl of lambda phosphatase buffer either in the presence or absence of lambda phosphatase (500 U, New England BioLabs), followed by incubation at 30°C for 1 h.

Briefly, each sample was normalized to contain equal amount of protein and the proteins were denatured and reduced in 10mM DTT containing 0.1% Rapigest (Waters, USA) at 50°C for 30 min. Subsequently, the reduced cysteines were alkylated with 30mM iodoacetamide for 1hr in the dark and the samples were digested overnight at 37°C using sequencing grade trypsin (1∶100,trypsin∶total protein).

For a fair comparison, all sequence datasets were randomly down-sampled (normalized) to contain reads representing a 15x average genome coverage.

Samples were normalized to contain equal concentrations of amplicons, combined and pair-end sequenced (250-nt reads) on the Illumina MiSeq by the Genomic Sequencing and Analysis Facility at the University of Texas at Austin.

Total protein in the crude extract was quantified by the Bradford method and normalized to contain ∼10 mg protein in a 5-ml volume of the sample before GFP-Trap affinity purification (Chromotek) using 35 μl of GFP-Trap agarose beads and processed for TiO2 phosphopeptide enrichment and mass spectrometry as previously described (12).

To enable comparison, we equalized individual assemblies of Normalized and Non-Normalized samples to contain the same number of base pairs before assembly.

The latter were either normalized [ 20] to contain one representative of each expressed gene of the whole genome (ORFeome) or derived from a mixed stage worm population.

Samples normalized by O.D. to contain about 10 cells of each culture were transferred to a microcentrifuge tube and centrifuged for 2 min at 14,000 ×  g.

The absorbance of each well was read at 570 nm and normalized to wells containing no cells or added probe (measured in quadruplicate).

The tab 'metabolites normalized to wt' contains the relative metabolite levels, which were used to calculate the fold changes shown in Figure 7E, as well as p-values from statistical tests.