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Secondly, 55 individual data regarding awake cortical resection of the eloquent cortices were integrated on the spatially normalized template.
These 2 linear transformations were then concatenated (GEMS → native space MRI → MNI) to derive a transformation between each subject's native EPI space and the normalized template space.
Each q-real-time PCR reaction (in 20 μl) contained 10 μl of Fast EvaGreen® Master Mix, 0.5 μl of each primer, 2 μl of normalized template cDNA, and 7 μl water.
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The ALE results were registered on an MNI-normalized template (http://brainmap.org/) using Mricro (http://www.mccauslandcenter.sc.edu/mricro/index.html).html
Normalized DNA template was quantified by real-time PCR as described previously (Ammar et al. 2009; Smith et al. 2009), diluted to 8pM, and clusters were generated on a single-read flow cell.
Next-generation sequencing: we used the Illumina HiSeq2000 sequencing platform as described below: Normalized DNA template was quantified by real-time PCR, diluted to 8 pM, and clusters were generated on a single-read flow cell.
The normalized b0 templates were averaged and smoothed with a 6 mm full-width-at-half-maximum (FWHM) Gaussian kernel to yield the b0 template.
Next, each individual MRI scan was spatially normalized to this template using an affine transformation.
For SPM analysis, the metabolic images were spatially normalized using the template provided in the SPM 99 package and subsequently smoothed with a 16 mm isotropic Gaussian kernel.
First, an affine transformation (12-degrees of freedom) between the image to be normalized and the template is calculated to spatially remove rotation, translation and scaling differences between reconstructed volumes.
Segmented GM images from the late scans (time 1 and time 2 scans) were normalized to GM template in MNI space, and the deformation applied to the product images [34].
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