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Data were normalized, significance determined by ANOVA and fold change was calculated with the Partek Genomics Suite (Partek Inc., St . Louis MO).
Data were normalized, significance determined by ANOVA, and fold change calculated with the Partek Genomics Suite (Partek Inc., St . Louis MO, USA).
Data were normalized, significance determined by ANOVA, and fold change calculated with the Partek Genomics Suite (Partek Inc .. Gene annotation enrichment analysis was performed with DAVID v6.7 [ 30], GSEA [ 65], and IPA (Ingenuity® Systems http://www.ingenuity.com) for all significantly changed genes (≥ +1.5 and ≤ −1.5 fold, P < 0.01).
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Protein group entries with a normalized ratio significance B score of ≤ 0.05 or significance A score of ≤ 0.05 were retained for further analysis.
Significance was calculated for upper and lower UIR quintiles for orthologs with maintained size, and was normalized to significance for all the equivalent quintiles for all orthologs ignoring UIR conservation.
To verify if the data is normally distributed, we have applied a test known as Shapiro-Wilk test [39], and the values for recall, precision, and time metrics were considered normalized with a significance level of 99.9%.
The data were then first quantile normalized [65] and significance of differential expression was then assessed with the Wilcoxon rank sum test [66] Ten genes that were differentially expressed at 24 and 66 h as observed by the array analysis were selected for validation by quantitative RT-PCR.
Statistical significance of normalized data was tested for by single sample Student's t test.
Quantitative Real-time RT-PCR was analysed for significance with normalized, calibrated expression ratios determined by one-way ANOVA statistical test with Tukey and Tukey-Kramer post hoc multiple comparison algorithms.
Figure 7 shows the statistical significance of normalized cross-correlations of time-varying fMRI signals among the 27 ROIs listed in Table 1 (t-test across subjects and fMRI runs).
The same was repeated with the Lowess normalized data using a significance value of 0.01.
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