9) Normalization of pre-score into a normalized score, which we denoted as regulatory activity score (RAS).
Expression of cystic fibrosis transmembrane conductance regulatory protein (CFTR) (normalized to glyceraldehyde-3-phosphate dehydrogenase [GAPDH]) was significantly decreased ~twofold (P<0.05) in the ob/ob mice compared with the leans, whilst crypt depth was unchanged.
The normalized protein levels reflect the full regulatory influence of the inserted sequences, including effects on mRNA processing, mRNA transport, mRNA stability and translation.
To analyze TFBSs and 3'UTR regulatory elements, we contrasted the normalized effect coefficients of genes that had at least one conserved regulatory element with those of the rest of the genes.
Recently, Das et al developed another computational model to identify CAEs for tissue-specific alternative splicing, which uses the correlation of motif parameters together with gene-level normalized exon expression signals to identify splicing regulatory motifs [ 34].
The log2-normalized expression profile of 24 genes from the regulatory network is plotted in the following Figure 1.
Rats exposed to zinc but not to MSH showed modulation of genes that form either these ion channels or their regulatory proteins (apparent when zinc data were normalized to MSH).
In this paper we used the information annotated in Tractor_DB (a database of regulatory networks in gamma-proteobacteria) to calculate a normalized Site Orthology Score (SOS) that quantifies the conservation of a regulatory link across thirty genomes of this subclass.
When compared to untreated MS patients, FTY displayed normalized naïve and memory B-cell counts in the CSF, whereas regulatory B-cell counts were retained (Fig. 4E).
