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All the micro-array experiments were performed in our laboratory with a normalized protocol of labeling, hybridization, data normalization and statistical analysis ensuring a perfect homogeneity of the data.
Invertebrates were sampled at 114 sites including reference and impacted river reaches following a normalized protocol.
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All isolates were identified according to normalized protocols [14].
Differential expression between D. miranda males and females was calculated for both species using the log2 fold change on the normalized expression counts using the normalization protocol implemented in the R package DESeq (Anders and Huber, 2010) version 1.10.1.
The DNA copy number of one clone, BAC57_O15 (8q21.13; BAC start 82359567 and end 82463826: NCBI homo sapiens genome Build 35.1) was assayed according to a previously described protocol with normalized normal human pooled genomic DNA (Promega, Madison, WI, USA) as a calibration sample.
In order to further observe the effect of power splitting coefficient ρ and energy harvesting time ratio α on the two protocols, Figure 4 shows the normalized throughput of two protocols, which is got by Equation 28. Figure 4 demonstrates that both PSR and TSR has the value of ρ and α to get maximum normalized throughput.
These datasets were all measured on Human Genome HG U133A Affymetrix arrays and normalized using the same protocol.
The protocol to normalized read counts of RNA-seq Illumina reads has been previously described [ 1].
Moreover, data are pre-processed and normalized following a specific protocol from a particular laboratory, instead of having standardized pre-processing and normalization workflows (Woodall and Goldberg 2008 ).
The NTSB also accused Enbridge of having a "culture of deviance," stating in a press release that"personnel had developed an operating culture in which not adhering to approved procedures and protocols was normalized".
The gene-expression data were normalized according to Agilent protocols.
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